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Image Search Results
Journal: Molecular Pain
Article Title: Involvement of TRPA1 activation in acute pain induced by cadmium in mice
doi: 10.1186/1744-8069-9-7
Figure Lengend Snippet: Cd-induced [Ca 2+ ] i increases in RIN-14B rat pancreatic cancer cells. ( A ) Actual traces of [Ca 2+ ] i responses to Cd (30 μM, 4 min) in RIN-14B cells. ( B ) Effect of removal of extracellular Ca (Ca0) and ( C ) HC-030031 (10 μM) on the Cd-induced [Ca 2+ ] i increases. Extracellular Ca was removed or a TRPA1 blocker was applied before and during 2 min application of Cd. ( D ) Summarized effects of these treatments. Columns show the increments of [Ca 2+ ] i in response to Cd. Symbols with vertical lines show mean ± SEM. (Control; n=33, Ca0; n=72, HC-030031; n=34, from 2 experiments) **,P <0.01.
Article Snippet: The
Techniques:
Journal: Cytotechnology
Article Title: Antidiabetic effect of green rooibos ( Aspalathus linearis ) extract in cultured cells and type 2 diabetic model KK-A y mice
doi: 10.1007/s10616-014-9816-y
Figure Lengend Snippet: Effect of GRE and aspalathin on glucose uptake, AMPK and Akt phosphorylation, and GLUT4 translocation in L6 myotubes. a–c Glucose uptake assay was conducted without or with GRE (0–800 µg/ml) or aspalathin (50 µM) and compound C (10 µM). Each value represents the mean ± SEM for 6 wells. Values not sharing a common letter are significantly different at P < 0.05 by Tukey–Kramer multiple comparisons test. d–f Western blotting analysis for AMPK and Akt phosphorylation and translocation of GLUT4 to the plasma membrane. Western blotting was conducted with anti-phospho-AMPK, anti-AMPK, anti-phopho-Akt, anti-Akt, anti-GLUT4 and anti-Na+K+-ATPase antibodies. Na+K+-ATPase is a marker enzyme of plasma membrane. PM plasma membrane fraction; Post-PM post-plasma membrane fraction. The bar diagramme in f shows the GLUT4/Na+K+-ATPase ratio of PM fraction to demonstrate the translocation of GLUT4 to the plasma membrane
Article Snippet: Keywords: AMPK, GLUT4, Hyperglycemia,
Techniques: Phospho-proteomics, Translocation Assay, Western Blot, Clinical Proteomics, Membrane, Marker
Journal:
Article Title: Type III InsP 3 receptor channel stays open in the presence of increased calcium
doi: 10.1038/23954
Figure Lengend Snippet: Ca 2+ transients in RIN-5F cells after stimulation by ATP
Article Snippet: We thank E. Kaftan for all his assistance during the execution of these experiments, J.
Techniques: